rabbit anti tim23  (Proteintech)

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Susceptibility of Mitophagy-Deficient Tumors to Ferroptosis Induction by Relieving the Suppression of Lipid Peroxidation.

doi: 10.1002/advs.202412593

Figure Lengend Snippet: Figure 2. Mitophagy-deficient cancer cells are sensitive to ferroptosis inducers. A,F) Immunoblot showing the expression of PINK1 in the indicated HCT116 cells (A) or MDA-MB-231 cells (F). B–E) Cell death and lipid peroxidation measurement in the indicated HCT116 cells treated with 12 μm erastin for 26 h (B,C) or cystine deprivation for 19 h (D,E). G–J) Cell death and lipid peroxidation measurement in the indicated MDA-MB-231 cells treated with 2 μm erastin for 12 h (G, H) or cystine deprivation for 7 h (I,J). K) Immunoblot showing the expression of Tom20 or Tim23 in HCT116 cells treated with control or mitophagy inbibitor 10 μm Mdivi-1 for 18 h. (L-O) Cell death and lipid peroxidation measurement in the indicated HCT116 cells treated with 12 μm erastin for 26 h (L,M) or cystine deprivation for 19 h (N,O) with or or without 10 μm Mdivi-1 or 10 μm Lipro-1. P) Immunoblot showing the expression of Tom20 or Tim23 in MDA-MB-231 cells treated with control or 10 μm Mdivi-1 for 12 h. Q–T) Cell death and lipid peroxidation

Article Snippet: Antibodies to the following were used: Parkin (CST, 2132S; 1:1000), PINK1 (CST, 6946S; 1:1000), ATF4 (CST, 11815S; 1:2000), TOM20 (Santa Cruz, sc-17764; 1:1000), TIM23 (CST, 34822S; 1:2000), and Phospho-PERK (CST, 3179S; 1:1000).

Techniques: Western Blot, Expressing, Control

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Susceptibility of Mitophagy-Deficient Tumors to Ferroptosis Induction by Relieving the Suppression of Lipid Peroxidation.

doi: 10.1002/advs.202412593

Figure Lengend Snippet: Figure 3. Mitophagy is enhanced during the ferroptotic process in cancer cells. A) Immunoblot showing the expression of Tom20 or Tim23 in HCT116 cells treated with erastin at the indicated concentrations and times. Left, time, 28h. Right, concentration, 16 μm. B) The relative mitochondrial DNA (mtDNA) measurement in HCT116 cells treated with erastin at the indicated concentrations and times. Left, time, 28h. Right, concentration, 16 μm. C) Immunoblot showing the expression of mitophagy-related proteins in HCT116 cells treated with erastin at the indicated concentrations and times. Left, time, 28h. Right, concentration, 16 μm. D) QPCR showing the expression of Parkin mRNA in HCT116 cells treated with erastin at the indicated

Article Snippet: Antibodies to the following were used: Parkin (CST, 2132S; 1:1000), PINK1 (CST, 6946S; 1:1000), ATF4 (CST, 11815S; 1:2000), TOM20 (Santa Cruz, sc-17764; 1:1000), TIM23 (CST, 34822S; 1:2000), and Phospho-PERK (CST, 3179S; 1:1000).

Techniques: Western Blot, Expressing, Concentration Assay